Not known Factual Statements About flow cytometer
Not known Factual Statements About flow cytometer
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Facts-enriched characterization of mobile populations. Morphological information and facts from illustrations or photos can increase for the richness of flow cytometry data. By way of example, the determine demonstrates an if not common apoptosis assay employing Annexin V and PI, introducing cell imaging to characterize cells in Every inhabitants to reveal morphologically distinct capabilities. These insights could not happen to be attained from multiplex staining by itself.
Irrespective of whether you’re a newbie or an authority, discover our resources to progress your exploration and know-how in flow cytometry.
Incorporate acoustic focusing technology with significant-parameter Assessment for versatile spectral unmixing and standard workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating using a high-pace brightfield camera for simultaneous large throughput flow cytometry and substantial resolution brightfield imaging.
"An image isn't really fairly really worth 1000 dots, nevertheless it genuinely helps to see photographs. It is excellent to find out illustrations or photos that correlate with staining. One example is, AnnexinV-favourable cells that are morphologically unique than healthier cells, or CD14+ monocytes which can be larger and even more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it really is illuminating to find out the number of doublets slip via regular singlet gates, and wonderful to verify the standing of unpredicted double-good functions in my Investigation."
Pair your Attune flow cytometer having an autosampler for amplified workflow performance, and increase robotic integration for max throughput.
Dependable graphic good quality even at higher throughput Acoustic focusing as well as a superior-speed camera Merge to impression these T cells constantly at very low or significant flow charges. Conveniently modify focus and camera configurations to fulfill experimental specifications.
Early signs of this contamination experienced to begin with been dismissed as particles. Backgating to discover new populations. Backgating imaged cells over click here the Attune CytPix Flow Cytometer also helps you to use morphological functions to discover appealing subpopulations that could not be clear from flow cytometry knowledge by yourself.
Probably most significantly, these gatherings contain cells of Plainly distinct phenotypes which could lead to incorrect conclusions with regards to double beneficial situations (particularly in exceptional populations).
AI-driven automated picture Evaluation While using the Attune Cytometric Application translates function characteristics into unique morphology parameters that may be coupled with conventional fluorescence and scatter parameters to expose really reproducible populace changes and exceptional subsets.
We Formerly demonstrated the power of imaging Vehicle-T/Ramos mobile interactions. Enable’s have a look at just the population of best fascination, the double constructive functions, To find out more. We could now use prolonged morphological parameters (below, circularity and skewness of depth) to further more examine the functions of these populations and refine gating on these gatherings, growing data robustness.
Built-in dimensionality reduction equipment amplified resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to verify envisioned populace resolution in lysed whole human blood. Consumer can look at functions from Each and every cluster for Visible confirmation of mobile phenotypes.
No clean/no lyse leukocyte Investigation with imaging. Cells had been obtained from 24-hour-outdated blood by dilution in one mM EDTA ( Professional UX/UI allows customers to practice AI models for expanded apps. (A) The “Train Model” aspect presents annotation instruments and true-time product instruction precision.
Gating on these prolonged parameters permits you to quickly and accurately identify populations of curiosity and ensure gating technique with little or no handbook critique. The Attune application features access to point out-of-the-art machine Finding out Investigation tools within an expertly created user interface, which makes it exceptionally simple to operate.
The Attune Xenith cytometer is really a significant-parameter spectral analyzer, furnishing both equally spectral unmixing and conventional flow capabilities for complete and versatile cell Examination.
The Attune CytPix Flow Cytometer enables buyers to visualize samples in the course of acquisition, validate the precision of gating approaches, and guidance benefits with reliable documentation for publication reasons.
What gave the impression to be a homogeneous populace is really much more various—an Perception that ought to be viewed as when interpreting results.